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Spectrin, Alpha, beta 抗体

适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal Spe 1-2 unconjugated
产品编号 ABIN6655089
发货至: 中国
  • 抗原
    Spectrin, Alpha, beta
    适用
    宿主
    小鼠
    克隆类型
    单克隆
    应用范围
    Western Blotting (WB), ELISA
    交叉反应 (详细)
    Cross reactivity with Spectrin from other sources has not been determined.
    纯化方法
    Anti-Spectrin antibody was purified from mouse ascites by protein A chromatography.  This product reacts with human.
    免疫原

    Immunogen: Spectrin(alpha and beta) antibody was produced in mice by repeated immunizations with human erythrocyte spectrin.

    Immunogen Type: Other

    克隆位点
    Spe 1-2
    亚型
    IgG1
  • 应用备注

    Application Note: Anti-Spectrin is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~246-280 kDa corresponding to the appropriate cell lysate or extract.

    ELISA Dilution: 1:40,000

    Western Blot Dilution: 2-4 μg/mL

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Neutral PBS

    缓冲液

    Buffer: 1.2 % sodium acetate

    Stabilizer: 2 mg BSA

    0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • 抗原
    Spectrin, Alpha, beta
    别名
    Spectrin (alpha and beta)
    背景

    Synonyms: EL2, HPP, HS3, SPH3, SPTA, EL3, HS2, SPH2, HSPTB1, Erythroid alpha-spectrin

    Background: Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5. This antibody is suitable for researchers interested in cytoskeleton and transcriptoion factor research.

    Gene Name: SPTA1, SPTB

    基因ID
    6708
    NCBI登录号
    NP_003117
    UniProt
    P02549
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