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SMCR5 Protein (AA 1-140) (Strep Tag)

SMCR5 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
产品编号 ABIN3077235
发货至: 中国
  • 抗原
    SMCR5 (Smith-Magenis Syndrome Chromosome Region, Candidate 5 (Non-Protein Coding) (SMCR5))
    蛋白类型
    Recombinant
    产品特性
    AA 1-140
    宿主
    资源
    Tobacco (Nicotiana tabacum)
    标记
    This SMCR5 protein is labelled with Strep Tag.
    应用范围
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    序列
    MRRCLRVKTR RGQLGLASSC FEQHSCFSPR VNRILSAVQN TLCTGPSSQA PPQPPQASPP AAADHSRTPS LLASSHSASG GESLFQLYIA SLAWPQNCCV LESCRRIPLG GLSSMENRRP LLRKGRLLRG QIHHSQTNEL
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    纯化方法
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    纯度
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    SMCR5 (Smith-Magenis Syndrome Chromosome Region, Candidate 5 (Non-Protein Coding) (SMCR5))
    别名
    SMCR5
    别名
    NCRNA00034 Protein, Smith-Magenis syndrome chromosome region, candidate 5 (non-protein coding) Protein, SMCR5 Protein
    背景
    Smith-Magenis syndrome chromosomal region candidate gene 5 protein
    分子量
    15.3 kDa
    UniProt
    Q8TEV8
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