Normal 驴 Serum
BI, ELISA, ICC, IF, IHC
宿主: 驴
Quick Overview for Normal 驴 Serum (ABIN7232252)
抗原
Normal Serum
宿主
驴
应用范围
BioImaging (BI), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)
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原理
- Normal Donkey Serum
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纯化方法
- Lipid extracted and dialyzed against 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2
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纯度
- Whole serum
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-p (1:10-20), IF/ICC (1:10-20) and ELISA (1:100).
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说明
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Blocking Serum
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 10 mM sodium phosphate, 0.15 M sodium chloride with 0.05 % sodium azide as preservative, pH 7.2
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 2-8°C or aliquot and store at or below -20°C for long-term storage. Avoid repeated freezing and thawing.
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- Normal Serum
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背景
- High quality Normal donkey serum processed from non-immunized normal adult donkeys is used as a blocking agent or as a negative control for immunoassays.,Normal donkey serums, which are from non-immuned donkey hosts, are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide. Normal serum diluted to 5 % -20 % (v/v) in PBS is strongly recommended as a blocking reagent to reduce background from non-specific binding, or control for most immunoassay applications. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.,Serum
抗原
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