兔 anti-Llama IgG (Chain gamma), (Heavy & Light Chain) Antibody (HRP)
ELISA, IHC, WB
HRP
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- 抗原 See all IgG products
- IgG
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抗原表位
- Chain gamma, Heavy & Light Chain
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适用
- Llama
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宿主
- 兔
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标记
- HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- Based on IEP, this antibody reacts with: heavy (gamma) chains on llama IgG, light chains on all llama immunoglobulins
- 无交叉反应
- Llama
- 纯化方法
- Affinity purified using solid phase Llama IgG (H and L)
- 纯度
- > 95 % based on SDS-PAGE
- 免疫原
- Purified Llama IgG, whole molecule
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- 应用备注
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This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:5,000 for Immunohistochemistry. 1:200-1:5000 for ELISA/Western blot - 说明
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Country of Origin: Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Rehydrate with 1.1 mL of deionized water. Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution. If necessary, Centrifuge to remove any particulates. Prepare fresh working dilution daily.
- 浓度
- 1.0 mg/mL
- 缓冲液
- 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
- 储存液
- Kathon CG
- 注意事项
- Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- 储存条件
- 4 °C
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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