兔 anti-绵羊 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed
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- 抗原 See all IgG products
- IgG
- 片段
- Fab fragment
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抗原表位
- Heavy & Light Chain
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适用
- 绵羊
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum.
- 产品特性
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 纯化方法
- Preadsorption: Solid phase absorption
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Sheep IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 1.2
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Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (FITC) - Preadsorbed
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Donkey anti-Sheep IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC Fab fragment
Rabbit anti-Sheep IgG (F(ab')2 Region) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Rabbit anti-Sheep IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFM Polyclonal IgG TRITC F(ab')2 fragment
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- 应用备注
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 说明
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Excitation/Emission wavelength: 494 nm/514 nm
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 0.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Rabbit Fab Anti-Sheep IgG Antibody Fluorescein Conjugation, Rabbit Fab Anti-Sheep IgG FITC Conjugated Antibody
Background: Fab Anti-Sheep IgG (H&L) Antibody generated in rabbit detects sheep IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Fab Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This Fab Anti-sheep IgG Antibody is conjugated to fluorescein.
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