山羊 anti-人 IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed
-
- 抗原 See all IgG products
- IgG
- 片段
- Fab fragment
-
抗原表位
- Heavy & Light Chain
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- TRITC
-
应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.
- 产品特性
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
-
Immunogen: Human IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 1.2
-
Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Mouse anti-Human IgG (Fc Region) antibody (PE)ELISA, FACS Monoclonal JDC-10 IgG1 PE
Mouse anti-Human IgG (Fab Region) antibody (PE)FACS Monoclonal 4A11 IgG2b PE
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Goat anti-Human IgG (Fc Region) antibody (PE) - PreadsorbedFACS, FM Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgG (Fc Region) antibody (Alkaline Phosphatase (AP))ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Human IgG (Fd Region) antibody (FITC)ELISA, FLISA Polyclonal IgG FITC
Goat anti-Human IgG antibody (HRP) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Human IgG (Fd Region) antibody (HRP)ELISA, FLISA Polyclonal IgG HRP
-
- 应用备注
-
Application Note: Fab Anti-Human IgG (H&L) Rhodamine Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store the vial at -20 °C or below after dilution.
- 有效期
- 12 months
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
-
Synonyms: Goat Fab Anti-Human IgG Antibody Rhodamine Conjugation, Goat Fab Anti-Human IgG TRITC Conjugated Antibody
Background: Fab Anti-Human IgG (H&L) Rhodamine Antibody generated in goat detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
-
