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兔 anti-小鸡 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin Fab fragment
产品编号 ABIN965310
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    Fab fragment
    抗原表位
    • 2381
    • 512
    • 424
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    • 95
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    • 22
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    • 2
    • 2
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 724
    • 699
    • 602
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    小鸡
    宿主
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    克隆类型
    • 3434
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    多克隆
    标记
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    Biotin
    应用范围
    • 1952
    • 1838
    • 1806
    • 1519
    • 934
    • 910
    • 652
    • 478
    • 380
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Rabbit Serum.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Chicken IgG whole molecule

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    0.5 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid cycles of freezing and thawing.
    This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store the vial at -20 °C or below after dilution.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Rabbit Fab Anti-Chicken IgG Biotin Conjugated Antibody, Rabbit Fab Fragment Anti-Chicken IgG Antibody Biotin Conjugation

    Background: Fab Anti-Chicken IgG Biotin Antibody generated in rabbit detects chicken IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.

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