山羊 anti-小鸡 IgG (Heavy & Light Chain) Antibody (Biotin)
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- 抗原 See all IgG products
- IgG
- 片段
- Fab fragment
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抗原表位
- Heavy & Light Chain
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适用
- 小鸡
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 原理
- Fab Chicken IgG (H&L) Antibody Biotin Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
- 产品特性
- Fab Anti-Chicken IgG Peroxidase Antibody generated in goat detects chicken IgY. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
- 免疫原
- Optional[Immunogen]: Chicken IgG whole molecule
- 亚型
- IgG
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Goat anti-Chicken IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
Donkey anti-Chicken IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedWB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Donkey anti-Chicken IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedWB, FLISA, FM Polyclonal IgG TRITC
Rabbit anti-Chicken IgG (F(ab')2 Region) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Chicken IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Chicken IgG (Heavy & Light Chain) antibodyELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
- Application Note: Fab Anti-Chicken IgG Biotin Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:80,000 - 1:800,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Fab Anti-Chicken IgG Biotin Antibody generated in goat detects chicken IgY. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present.
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