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驴 anti-绵羊 IgG (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin F(ab')2 fragment
产品编号 ABIN965291
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    F(ab')2 fragment
    抗原表位
    • 2271
    • 457
    • 363
    • 188
    • 130
    • 123
    • 92
    • 29
    • 28
    • 28
    • 17
    • 9
    • 6
    • 6
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    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 685
    • 684
    • 567
    • 436
    • 378
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    • 2
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    • 1
    • 1
    • 1
    • 1
    • 1
    绵羊
    宿主
    • 1678
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    • 104
    • 90
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    • 1
    克隆类型
    • 3419
    • 196
    • 1
    多克隆
    标记
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    • 15
    Biotin
    应用范围
    • 1777
    • 1700
    • 1686
    • 1440
    • 934
    • 910
    • 883
    • 463
    • 243
    • 243
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    • 100
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    • 50
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Donkey Serum, Sheep IgG and Sheep Serum.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Sheep IgG whole molecule

    亚型
    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:2,500

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    ELISA Dilution: 1:10,000 - 1:50,000

    Western Blot Dilution: 1:1,000 - 1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid cycles of freezing and thawing.
    This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Donkey F(ab')2 Anti-Sheep IgG Biotin Conjugated Antibody, Donkey Fab2 Anti-Sheep IgG Antibody Biotin Conjugation

    Background: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

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