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山羊 anti-兔 IgG (F(ab')2 Region) Antibody (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin F(ab')2 fragment
产品编号 ABIN965224
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    F(ab')2 fragment
    抗原表位
    • 2333
    • 513
    • 418
    • 206
    • 132
    • 130
    • 98
    • 29
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    • 28
    • 22
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    • 1
    F(ab')2 Region
    适用
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    宿主
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    山羊
    克隆类型
    • 3387
    • 187
    • 1
    多克隆
    标记
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    Biotin
    应用范围
    • 1960
    • 1822
    • 1799
    • 1520
    • 916
    • 893
    • 650
    • 478
    • 380
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    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
    交叉反应
    产品特性
    F(ab')2 anti-Rabbit IgG antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Anti-Rabbit IgG F(ab')2 fragment was produced by repeated immunization with Rabbit IgG whole molecule in goat.

    Immunogen Type: Native Protein

    亚型
    IgG
    Labeling Ratio
    10-20
  • 应用备注

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: F(ab')2 anti-Rabbit IgG is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid repeated freezing and thawing.
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Goat F(ab')2 Anti-Rabbit IgG biotin conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody biotin Conjugation

    Background: F(ab')2 anti-Rabbit IgG antibody generated in goat detects specifically Rabbit IgG whole molecule. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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