山羊 anti-兔 IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed
-
- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
-
抗原表位
- Heavy & Light Chain
-
适用
- 兔
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- PE
-
应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
- 纯化方法
- Preadsorption: immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
- 免疫原
-
Immunogen: Rabbit IgG whole molecule
- 亚型
- IgG
-
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC) - Preadsorbed
FACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 425
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 488) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 488
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 647N
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 594) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 594
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC F(ab')2 fragment
-
- 应用备注
-
Application Note: F(ab')2 Anti-Rabbit IgG Phycoerythrin Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
Flow Cytometry Dilution: 1:100 - 1:250
IF Microscopy Dilution: 1:100 - 1:250
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 0.5 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Dilute only prior to immediate use
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20 °C or below after dilution. - 储存条件
- RT,4 °C
- 储存方法
- Store vial at -20 °C or below prior to opening.
- 有效期
- 12 months
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
-
Synonyms: Goat F(ab')2 Anti-Rabbit IgG Phycoerythrin Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody PE Conjugation
Background: F(ab')2 Anti-Rabbit IgG (H&L) Antibody generated in goat detects immunoglobulin g from Rabbit, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. This F(ab')2 Anti-Rabbit IgG Antibody is conjugated to Phycoerythrin.
-
