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山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Rockland 710-1332 ELISA, IHC, WB Polyclonal IgG HRP F(ab')2 fragment
Rockland
产品编号 ABIN965171
Supplier Product No.: 710-1332
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    F(ab')2 fragment
    抗原表位
    • 2402
    • 511
    • 417
    • 148
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
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    • 1
    • 1
    Heavy & Light Chain
    适用
    • 720
    • 691
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    • 393
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    小鼠
    宿主
    • 1760
    • 1549
    • 569
    • 238
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    山羊
    克隆类型
    • 3389
    • 175
    • 1
    多克隆
    标记
    • 583
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    HRP
    应用范围
    • 2032
    • 1981
    • 1835
    • 1784
    • 983
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    • 896
    • 486
    • 420
    • 234
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Supplier Product No.
    710-1332
    Supplier
    Rockland
    原理
    F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
    交叉反应 (详细)
    Minimal crossreactivity against Bv Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
    产品特性
    F(ab')2 Anti-Mouse IgG F(ab')2 Peroxidase Antibody generated in goat detects Mouse F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
    纯化方法
    Preadsorption: Pre-Adsorbed
    免疫原

    Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注
    Application Note: F(ab')2 anti-Mouse IgG (H&L) peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:300:000 Other: User Optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Nakayama, Sakashita, Nariai, Kato, Sinmyozu, Nakayama, Kyo, Urano, Nakayama: "Cancer-related transcription regulator protein NAC1 forms a protein complex with CARM1 for ovarian cancer progression." in: Oncotarget, Vol. 9, Issue 47, pp. 28408-28420, (2018) (PubMed).

    Tomiyama, Kamino, Fukamachi, Urano: "Precise epitope determination of the anti-vimentin monoclonal antibody V9." in: Molecular medicine reports, Vol. 16, Issue 4, pp. 3917-3921, (2018) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    F(ab')2 anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in goat detects specifically mouse IgG (H&L). This secondary conjugated antibody anti-mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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