山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed
(1 reference)-
- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- Heavy & Light Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG and Mouse Serum.
- 产品特性
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Mouse IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 2.8
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Goat anti-Mouse IgG antibody (DyLight 488) - Preadsorbed
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Rabbit anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
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Rabbit anti-Mouse IgG antibody (Atto 594) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 594
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
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- 应用备注
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Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 说明
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Excitation/Emission wavelength: 494 nm/514 nm
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Do NOT add Sodium Azide!
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
- 有效期
- 12 months
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: "Akt and c-Myc differentially activate cellular metabolic programs and prime cells to bioenergetic inhibition." in: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 7324-33, (2010) (PubMed).
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: "Akt and c-Myc differentially activate cellular metabolic programs and prime cells to bioenergetic inhibition." in: The Journal of biological chemistry, Vol. 285, Issue 10, pp. 7324-33, (2010) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat F(ab')2 Anti-Mouse IgG Antibody Fluorescein Conjugation, Goat Fab2 Anti-Mouse IgG FITC conjugated Antibody
Background: F(ab')2 Anti-Mouse IgG (H&L) Fluorescein Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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