山羊 anti-人 IgM (Fc5mu Region) Antibody (HRP) - Preadsorbed
-
- 抗原 See all IgM products
- IgM
- 片段
- F(ab')2 fragment
-
抗原表位
- Fc5mu Region
-
适用
- 人
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- HRP
-
应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgM and Human Serum.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
-
Immunogen: Human IgM Fc5mu fragment
- 亚型
- IgG
-
-
- 应用备注
-
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: F(ab')2 Anti-Human IgM Fc5μ Peroxidase Antibody is designed for Western blot and ELISA.
ELISA Dilution: 1:20,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. - 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
- 有效期
- 12 months
-
- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
-
Synonyms: Goat F(ab')2 Anti-Human IgM Fc5μ Antibody Peroxidase Conjugation, Goat Fab2 Anti-Human IgM Fc5μ HRP Conjugated Antibody
Background: F(ab')2 Anti-Human IgM Fc5μ Peroxidase Antibody generated in goat detects specifically the Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
-