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山羊 anti-人 IgM (Fc5mu Region) Antibody (FITC) - Preadsorbed

FACS, FM Polyclonal IgG FITC F(ab')2 fragment
产品编号 ABIN965101
发货至: 中国
  • 抗原 See all IgM products
    IgM
    片段
    F(ab')2 fragment
    抗原表位
    • 162
    • 123
    • 87
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    • 25
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    • 1
    Fc5mu Region
    适用
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    宿主
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    山羊
    克隆类型
    • 533
    • 88
    多克隆
    标记
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    FITC
    应用范围
    • 375
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    Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgM and Human Serum.
    产品特性
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Human IgM Fc5mu  fragment

    亚型
    IgG
    Labeling Ratio
    3.7
  • 应用备注

    Application Note: F(ab')2 Anti-Human IgM Fc5μ Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Flow Cytometry Dilution: 1:500-1:2,500

    IF Microscopy Dilution: 1:500 - 1:2,500

    说明

    Excitation/Emission wavelength: 494 nm/514 nm

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    Avoid cycles of freezing and thawing.
    Do NOT add Sodium Azide!
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
    有效期
    12 months
  • 抗原
    IgM
    Abstract
    IgM 产品
    物质类
    Antibody
    背景

    Synonyms: Goat F(ab')2 Anti-Human IgM Fc5μ Antibody Fluorescein Conjugation, Goat Fab2 Anti-Human IgM Fc5μ FITC Conjugated Antibody

    Background: F(ab')2 Anti-Human IgM Fc5μ Fluorescein Antibody generated in goat detects specifically the Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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