山羊 anti-人 IgM (Fc5mu Region) Antibody (FITC) - Preadsorbed
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- 抗原 See all IgM products
- IgM
- 片段
- F(ab')2 fragment
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抗原表位
- Fc5mu Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgM and Human Serum.
- 产品特性
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Human IgM Fc5mu fragment
- 亚型
- IgG
- Labeling Ratio
- 3.7
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- 应用备注
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Application Note: F(ab')2 Anti-Human IgM Fc5μ Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Flow Cytometry Dilution: 1:500-1:2,500
IF Microscopy Dilution: 1:500 - 1:2,500
- 说明
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Excitation/Emission wavelength: 494 nm/514 nm
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
Do NOT add Sodium Azide!
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat F(ab')2 Anti-Human IgM Fc5μ Antibody Fluorescein Conjugation, Goat Fab2 Anti-Human IgM Fc5μ FITC Conjugated Antibody
Background: F(ab')2 Anti-Human IgM Fc5μ Fluorescein Antibody generated in goat detects specifically the Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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