山羊 anti-人 IgM Antibody (FITC)
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- 抗原 See all IgM products
- IgM
- 片段
- F(ab')2 fragment
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Fluorescence Microscopy (FM)
- Supplier Product No.
- 709-1231
- Supplier
- Rockland
- 原理
- F(ab')2 Human IgM Fc5u Antibody Fluorescein Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.
- 产品特性
- F(ab')2 Anti-Human IgG F(ab')2 Fluorescein Antibody generated in goat detects F(ab')2 from human. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
- 免疫原
- Optional[Immunogen]: Human IgM Fc5mu fragment
- 亚型
- IgG
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Mouse anti-Human IgM antibody (FITC)
FACS Monoclonal CH2 IgG1 FITC
Mouse anti-Human IgM antibody (APC)FACS Monoclonal CH2 IgG1 APC
Mouse anti-Human IgM antibody (HRP)ELISA, WB Monoclonal CH2 IgG1 HRP
Goat anti-Human IgM antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Mouse anti-Human IgM antibody (PE)FACS Monoclonal CH2 IgG1 PE
Mouse anti-Human IgM antibody (iFluor™647)GMP Grade FACS Monoclonal M-2E6 IgG1 iFluor™647
Mouse anti-Human IgM antibody (mFluor™450)GMP Grade FACS Monoclonal M-2E6 IgG1 mFluor™450
Mouse anti-Human IgM antibody (PE-Cy7)GMP Grade FACS Monoclonal M-2E6 IgG1 PE-Cy7
Goat anti-Human IgM antibody (FITC)ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human IgM (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
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- 应用备注
- Application Note: F(ab')2 Anti-Human IgM Fc5μ Fluorescein Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500-1:2,500 IF Microscopy Dilution: 1:500 - 1:2,500 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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