山羊 anti-人 IgG (F(ab')2 Region) Antibody - Preadsorbed
(1 reference)-
- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
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抗原表位
- F(ab')2 Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(ab')2 and Human Serum.
- 交叉反应
- 人
- 产品特性
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Anti-Human antibody generated in goat detects specifically human IgG F(ab')2. This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Anti-Human antibody generated in goat detects specifically human IgG F(ab')2. This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. - 纯化方法
- Preadsorption: Solid phase absorption
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-Human IgG was produced by repeated immunization with human IgG F(ab')2 fragment in goat.
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibody (Biotin) - PreadsorbedELISA, IHC, WB Polyclonal IgG Biotin
Mouse anti-Human IgG (Fab Region) antibodyELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Goat anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP
Goat anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Mouse anti-Human IgG antibodyELISA, WB Monoclonal 4D2D9G8 IgG1 unconjugated
Goat anti-Human IgG (Fc Region) antibody (HRP)ELISA, WB Polyclonal HRP F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (PE)ELISA, FACS Monoclonal JDC-10 IgG1 PE
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- 应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Antibody Anti-Human IgG F(ab')2 is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:25,000
Western Blot Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid cycles of freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial antibody at 4 °C prior to restoration. For extended storage aliquot antibody and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
- 有效期
- 12 months
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: "Molecular mediators for raft-dependent endocytosis of syndecan-1, a highly conserved, multifunctional receptor." in: The Journal of biological chemistry, Vol. 288, Issue 20, pp. 13988-99, (2013) (PubMed).
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: "Molecular mediators for raft-dependent endocytosis of syndecan-1, a highly conserved, multifunctional receptor." in: The Journal of biological chemistry, Vol. 288, Issue 20, pp. 13988-99, (2013) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody Pre-Adsorbed, Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody
Background: F(ab')2 Anti-Human IgG F(ab')2 Antibody generated in goat detects F(ab')2 from human. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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