山羊 anti-兔 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed
(6 references)
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 611-1322-0100
- Supplier
- Rockland
- 原理
- Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
- 产品特性
- Anti-Rabbit IgG F(ab')2 Antibody generated in goat recognizes the dimeric Fab portion of the rabbit IgG molecule. Rabbit IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind rabbit IgG F(c) will not bind rabbit IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG F(ab')2 is conjugated to Peroxidase.
- 纯化方法
- Anti-RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
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Immunogen: Rabbit IgG whole molecule
Immunogen Type: Native Protein
- 亚型
- IgG
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Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&RUN, CUT&Tag Polyclonal IgG unconjugated
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC)WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)High-quality product from Rockland ELISA, IHC, WB, DB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 800)ELISA, WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 488)WB, IF, FLISA, FM, DB, MA Polyclonal IgG DyLight 488
Goat anti-Rabbit IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 550
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 647N
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- 应用备注
- Application Note: Goat Anti-Rabbit IgG peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:750 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:50,000 - 1:100,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "miR-22 Suppresses Tumor Invasion and Metastasis in Colorectal Cancer by Targeting NLRP3." in: Cancer management and research, Vol. 12, pp. 5419-5429, (2020) (PubMed).
: "Anterograde trafficking signals in GABAA subunits are required for functional expression." in: Channels (Austin, Tex.), Vol. 13, Issue 1, pp. 440-454, (2020) (PubMed).
: "Molecular features of steroid-binding antidins and their use for assaying serum progesterone." in: PLoS ONE, Vol. 14, Issue 2, pp. e0212339, (2019) (PubMed).
: "Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells." in: Oncotarget, Vol. 8, Issue 29, pp. 46781-46800, (2018) (PubMed).
: "Sustained submicromolar H2O2 levels induce hepcidin via signal transducer and activator of transcription 3 (STAT3)." in: The Journal of biological chemistry, Vol. 287, Issue 44, pp. 37472-82, (2013) (PubMed).
: "Apoptolysis: a novel mechanism of skin blistering in pemphigus vulgaris linking the apoptotic pathways to basal cell shrinkage and suprabasal acantholysis." in: Experimental dermatology, Vol. 18, Issue 9, pp. 764-70, (2009) (PubMed).
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: "miR-22 Suppresses Tumor Invasion and Metastasis in Colorectal Cancer by Targeting NLRP3." in: Cancer management and research, Vol. 12, pp. 5419-5429, (2020) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG is conjugated with Peroxidase. This peroxidase conjugated anti-Rabbit secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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