山羊 anti-兔 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed
(31 references)
(1 validation)-
- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 特异性
- IgG (H&L)
- 交叉反应
- 兔
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Rabbit IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 3.1
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Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 425
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 488) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 488
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 647N
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 594) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 594
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC F(ab')2 fragment
Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
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- 应用备注
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 说明
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Excitation/Emission wavelength: 494 nm/514 nm
- 限制
- 仅限研究用
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- by
- Okeanos Research Laboratory, Department of Biological Sciences, Clemson University
- No.
- #100071
- 日期
- 2016.09.14
- 抗原
- Rabbit IgG (Heavy & Light Chain)
- Lot Number
- 611-1202
- Method validated
- Immunofluorescence
- Positive Control
- Lab stock CBD-SNAP antibody
- Negative Control
- No SNAP-tag antibody
- Notes
- We validate the specificity of the secondary goat anti-rabbit IgG (heavy & light chain) antibody (FITC) ABIN101988 for rabbit IgG antibody.
- Primary Antibody
- ABIN1573927
- Secondary Antibody
- ABIN101988
- Full Protocol
- Oyster visceral mass tissue is dissected and fixed in 4% paraformaldehyde in seawater overnight.
- Serial dehydration process using an automated ASP300S Enclosed Tissue Processor (Leica Biosystems) as follows:
- 70% ethanol for 45min
- 90% ethanol for 45min
- 90% ethanol for 45min
- 100% ethanol twice for 45min
- xylene twice for 45min
- paraffin wax at 58°C 3 times for 30 min
- Tissue is mounted in a paraffin block and hardened overnight before.
- 8µm tissue sections are retrieved from the block and collected on circular glass cover slips.
- Heat cover slips at 60°C for 1h.
- Deparaffination and rehydration:
- Xylene twice for 15min
- 100% ethanol twice for 10min
- 95% ethanol for 10 min
- 85% ethanol for 10 min
- 70% ethanol for 10 min
- 50% ethanol for 10 min
- 30% ethanol for 10 min
- distilled water for 10 min
- PBS for 10 min
- Wash tissue sections with PBS with 0.05% triton X twice for 30min.
- Permeabilize in PBS with 0.05% triton X overnight.
- Treatment of the tissue sections with 1mg/mL sodium borohydride in PBS three times for 5min to reduce autofluorescence.
- Wash sections in PBS 3 times for 15 min for at RT.
- Block sections in PBST with 1% BSA for 2 hours at RT.
- Incubate sections with CBD-SNAP antibody (lab stock) diluted 1:200 in PBST with 1% BSA overnight at 4°C to detect the location of chitin.
- Wash sections in PBS 3 times for 15min with PBS at RT.
- Additionally, incubate the CBD-SNAP and SNAP-tag double-stained sections with rabbit anti-SNAP antibody (antibodies-online, ABIN1573927, lot 13D000621) diluted 1:200 in PBST with 1% BSA overnight at 4°C.
- Wash sections in PBS 3 times for 15min with PBS at RT.
- Incubate sections with the secondary goat anti-rabbit IgG (heavy & light chain) antibody (FITC) (antibodies-online, ABIN101988, lot 611-1202) diluted 1:400 in PBST with 1% BSA for 2h at °C.
- Wash sections in PBS three times for 15min at RT.
- Counterstain with 0.1µg/mL DAPI in PBS for 15min at RT.
- Wash sections in PBS three times for 15min at RT.
- Mount sections on a microscopic slide using 50% glycerol in PBS.
- Seal cover slips with nail polish.
- Confocal imaging on Leica SPE.
- Visualization of the data performed on LAS 3D software.
- Experimental Notes
- To validate the specificity of the anti-rabbit FITC secondary antibody ABIN101988, 8µm paraffin sections of oyster visceral mass were observed in this study. We compared the fluorescence signals with immunofluorescence study. The negative control specimen was always compared with the test specimen or the positive control specimen on the same day, using the same laser power, gain, offset, accumulation/averaging settings on the Leica SPE confocal microscope. Visualization of the data was performed on LAS 3D software, with the same visualization setting to compare signal brightness. We found that the samples treated with anti-rabbit FITC secondary antibody ABIN101988 had similar fluorescence signals as the positive control Anti Rabbit Alexa 488. Excitation at the same laser wavelength and power did not generate fluorescence in the negative control section, when anti-rabbit FITC secondary antibody was applied in the absence of rabbit produced anti-SNAP antibody.
生效 #100071 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Immunofluorescence images of oyster visceral mass tissue, with the specificity of Anti-Rabbit Alexa 488 (top row) and Anti-Rabbit FITC (bottom row) compared. Unstained samples (left column) were compared to test samples (right column) to visualize degree of auto-fluorescence. The absence of anti-SNAP served as the negative control (middle column), it has led to the absence of fluorescence at the same imaging and visualization setting for the green channels, implying the goat anti-rabbit IgG (Heavy & Light Chain) antibody FITC conjugate ABIN101988 is specific to Rabbit IgG antigen.]()
Immunofluorescence images of oyster visceral mass tissue, with the specificity of Anti-Rabbit Alexa 488 (top row) and Anti-Rabbit FITC (bottom row) compared. Unstained samples (left column) were compared to test samples (right column) to visualize degree of auto-fluorescence. The absence of anti-SNAP served as the negative control (middle column), it has led to the absence of fluorescence at the same imaging and visualization setting for the green channels, implying the goat anti-rabbit IgG (Heavy & Light Chain) antibody FITC conjugate ABIN101988 is specific to Rabbit IgG antigen.
Full Methods -
- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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: "LncRNA Neat1 Promotes Regeneration after Spinal Cord Injury by Targeting miR-29b." in: Journal of molecular neuroscience : MN, Vol. 71, Issue 6, pp. 1174-1184, (2021) (PubMed).
: "Effects of sevoflurane general anesthesia during early pregnancy on AIM2 expression in the hippocampus and parietal cortex of Sprague-Dawley offspring rats." in: Experimental and therapeutic medicine, Vol. 21, Issue 5, pp. 469, (2021) (PubMed).
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: "MicroRNA-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating FOS expression." in: PloS one, Vol. 16, Issue 8, pp. e0255480, (2021) (PubMed).
: "Klotho Inhibits Proliferation in a RET Fusion Model of Papillary Thyroid Cancer by Regulating the Wnt/β-Catenin Pathway." in: Cancer management and research, Vol. 13, pp. 4791-4802, (2021) (PubMed).
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: "miR-135a inhibits airway inflammatory response in asthmatic mice via regulating JAK/STAT signaling pathway." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 54, Issue 3, pp. e10023, (2021) (PubMed).
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: "Association between dietary selenium intake and the prevalence of osteoporosis and its role in the treatment of glucocorticoid-induced osteoporosis." in: Journal of orthopaedic surgery and research, Vol. 18, Issue 1, pp. 867, (2023) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: Goat anti-Rabbit IgG Antibody fluorescein Conjugation, Goat anti-Rabbit IgG FITC Conjugated Antibody
Background: Anti-Rabbit IgG Antibody Fluorescein generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to Fluorescein.
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