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- 抗原 See all IgG products
- IgG
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适用
- 山羊
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宿主
- 驴
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克隆类型
- 多克隆
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标记
- PE
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Dot Blot (DB)
- 原理
- Goat IgG Antibody Phycoerythrin conjugated Pre-Adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
- 产品特性
- Anti-Goat IgG Biotin Antibody generated in donkey detects goat IgG.
- 纯化方法
- Anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- 免疫原
- Optional[Immunogen]: Goat IgG whole molecule
- 亚型
- IgG
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Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 550) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 550
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 532) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 532
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB, DB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Atto 594) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 594
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Donkey anti-Goat IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Donkey anti-Goat IgG (Heavy & Light Chain) antibody (Biotin)ELISA, IHC, WB Polyclonal IgG Biotin Fab fragment
Chicken anti-Goat IgG antibody (DyLight 550)WB, IF Polyclonal IgY DyLight 550
Donkey anti-Goat IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedWB, FACS, FLISA, FM Polyclonal IgG Texas Red (TR)
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- 应用备注
- Application Note: Anti-Goat IgG Phycoerythrin Conjugated antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Specific conditions for reactivity should be optimized by the end user. Flow Cytometry Dilution: 1:100 - 1:250 Western Blot Dilution: 1:1,000 IF Microscopy Dilution: 1:100 - 1:250 Other: FLOW 1:100 - 1:250
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 0.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
- 有效期
- 6 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Anti-Goat IgG (H&L) antibody is ideal for investigators involved in serum component protein research.
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