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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 特异性
- IgG (H&L)
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 纯化方法
- Anti-Chicken IgG/IgY Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins.
- 免疫原
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Immunogen: Chicken IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 3.0
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Donkey anti-Chicken IgG (Heavy & Light Chain) antibody (TRITC) - Preadsorbed
FLISA, FM Polyclonal IgG TRITC
Donkey anti-Chicken IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Chicken IgG (Heavy & Light Chain) antibodyELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Goat anti-Chicken IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Rabbit anti-Chicken IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
Rabbit anti-Chicken IgG (F(ab')2 Region) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Rabbit anti-Chicken IgG (Fc Region) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
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- 应用备注
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Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 说明
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Excitation/Emission wavelength: 494 nm/514 nm
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.5 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Product is photosensitive and should be protected from light.
- 储存条件
- RT,4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
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Synonyms: rabbit anti-Chicken IgG Antibody Fluorescein Conjugation, rabbit anti-Chicken IgG FITC Conjugated Antibody
Background: Anti-Chicken IgG Fluorescein Antibody generated in rabbit detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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