兔 anti-Llama VHH Antibody
ELISA, WB
Polyclonal
IgG
unconjugated
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- 抗原
- VHH
- 适用
- Llama
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宿主
- 兔
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克隆类型
- 多克隆
- 应用范围
- ELISA, Western Blotting (WB)
- 原理
- Rabbit Anti-VHH antibody can be used to detect single domain VHH antibodies.
- 交叉反应 （详细）
- Anti-VHH antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3.
- 产品特性
- Rabbit Anti-VHH antibody can be used to detect single domain VHH antibodies. The single-domain antibody (sdAb) is a small (12 - 14 kDa) antibody fragment that consists of a monomeric variable domain derived from the heavy chain, also called a VHH antibody. These heavy chain only Fab-like domains have activity that is similar to a whole antibody, and they are able to bind to a specific antigens. SdAb's are derived from camelid species that include llamas, alpacas and camels. Camelids produce both classical (containing heavy and light chain fragments) and non-classical antibody structures (containing only a heavy chain). VHH antibodies are the smallest functional antigen-binding fragment that occurs in nature and these are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and they have potential for therapeutic activity.
- 纯化方法
- Anti-VHH is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VHH camelid domain protein.
Immunogen Type: Native Protein
- 亚型
- IgG
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- 应用备注
- Application Note: Anti-VHH antibody has been tested by ELISA and Western blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed. Western Blot Dilution: 1:1,000-1:5,000 ELISA Dilution: 1:10,000-1:50,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 80 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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- 抗原
- VHH
- 背景
- The single-domain antibody (sdAb) is a small (12 - 14 kDa) antibody fragment that consists of a monomeric variable domain derived from the heavy chain, also called a VHH antibody. These heavy chain only Fab-like domains have activity that is similar to a whole antibody, and they are able to bind to a specific antigens. SdAb's are derived from camelid species that include llamas, alpacas and camels. Camelids produce both classical (containing heavy and light chain fragments) and non-classical antibody structures (containing only a heavy chain). VHH antibodies are the smallest functional antigen-binding fragment that occurs in nature and these are now being used in biotechnology as a novel antibody scaffold. The small size of the VHH single domain antibody makes it very attractive for use in diagnostic imaging and they have potential for therapeutic activity.
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