ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Dot Blot (DB), Multiplex Assay (MA)
Supplier Product No.
612-4202-1.0
Supplier
Rockland
原理
Rabbit Anti-Rat IgG H&L (FITC)
特异性
IgG (H&L)
纯化方法
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Rat IgG and Rat Serum.
Anti-Rat IgG Fluorescein conjugated antibody has been tested by dot blot. Anti-Rat IgG FITC conjugated secondary antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
限制
仅限研究用
状态
Lyophilized
溶解方式
Restore with deionized water (or equivalent)
浓度
2.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1