ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
原理
Rabbit Anti-Goat IgG H&L (HRP)
特异性
IgG (H&L)
纯化方法
Rabbit Anti-Goat IgG secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum.
Anti-Goat IgG Peroxidase conjugate has been tested by ELISA and western blot. This antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
限制
仅限研究用
状态
Lyophilized
溶解方式
Restore with deionized water (or equivalent)
浓度
2.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
储存液
Gentamicin sulfate
注意事项
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件
4 °C,-20 °C
储存方法
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1