ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
Supplier Product No.
609-1302-1.0
Supplier
Rockland
原理
Goat Anti-Human IgG H&L (HRP)
特异性
IgG (H&L)
纯化方法
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG and Human Serum.
质量等级
High-quality product from Rockland
免疫原
Anti-Human IgG (H&L) was produced by repeated immunization with human IgG whole molecule in goat. Immunogen Type: Native Protein
Anti-Human IgG (H&L) peroxidase conjugated antibody has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
限制
仅限研究用
状态
Lyophilized
溶解方式
Restore with deionized water (or equivalent)
浓度
2.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Gentamicin Sulfate, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
储存液
Gentamicin sulfate
注意事项
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件
4 °C,-20 °C
储存方法
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
有效期
12 months
Jian, Xiujian, Yuangang, Yan, Lianchao, Duthie, Yan: "Evaluation of antibody detection against the NDO-BSA, LID-1 and NDO-LID antigens as confirmatory tests to support the diagnosis of leprosy in Yunnan province, southwest China." in: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 114, Issue 3, pp. 193-199, (2021) (PubMed).
Phan, Subramanian, Kim, Murphy, Pettie, Carter, Anishchenko, Barrett, Craig, Tillery, Shek, Harrington, Koelle, Wald, Veesler, King, Boonyaratanakornkit, Isoherranen, Greninger, Jerome, Chu, Staker et al.: "In silico detection of SARS-CoV-2 specific B-cell epitopes and validation in ELISA for serological diagnosis of COVID-19. ..." in: Scientific reports, Vol. 11, Issue 1, pp. 4290, (2021) (PubMed).
Huang, Tan, Chen, Huang, Harvey, Hussain, Chen, Harding, Gilbert-Jaramillo, Liu, Knight, Schimanski, Shih, Lin, Cheng, Cheng, Huang, Lin, Jan, Ma, James, Daniels, McCauley, Rijal, Townsend: "Breadth and function of antibody response to acute SARS-CoV-2 infection in humans." in: PLoS pathogens, Vol. 17, Issue 2, pp. e1009352, (2021) (PubMed).
Serrano-Coll, Muñoz, Camilo Beltrán, Duthie, Cardona-Castro: "Anti-natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) antibodies as indicators of leprosy reactions and neuritis." in: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 111, Issue 3, pp. 125-131, (2018) (PubMed).
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1