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Recombinant Alpaca anti-小鼠 IgG2 Antibody (Biotin)

WB, IHC, ICC, IF, FACS Monoclonal 14A4 Recombinant Antibody Biotin single-domain Antibody (sdAb)
产品编号 ABIN7272948
发货至: 中国
  • 抗原 See all IgG2 products
    IgG2
    抗体类型
    Recombinant Antibody
    片段
    single-domain Antibody (sdAb)
    适用
    • 22
    • 12
    • 7
    • 7
    • 6
    • 1
    小鼠
    宿主
    • 19
    • 11
    • 10
    • 10
    • 5
    Alpaca
    Expression System
    E.coli
    克隆类型
    • 31
    • 24
    单克隆
    标记
    • 21
    • 8
    • 8
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Biotin
    应用范围
    • 29
    • 27
    • 24
    • 20
    • 14
    • 8
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
    特异性
    Mouse IgG2, does not cross-react with rabbit, guinea pig or chicken immunoglobulins
    纯化方法
    Produced in: E.coli
    免疫原
    sdAb anti-Mouse IgG2a/b binds to mouse-IgG2a and IgG2b subtype specifically and with high affinity. anti-mouse IgG2 sdAb binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain.
    克隆位点
    14A4
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconsitute with 250 µL pure H20 for immidate use or with 250 µL 50 % glycerol for storage at -80 °C
    缓冲液
    sdAbs are lyophilized from PBS pH 7.4.
    储存条件
    4 °C/-20 °C/-80 °C
    储存方法
    Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
  • 抗原
    IgG2
    Abstract
    IgG2 产品
    物质类
    Antibody
    背景
    Most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).
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