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Recombinant 小鼠 anti-人 IgG Antibody (HRP)
ELISA, WB, DB
Monoclonal
Recombinant Antibody
HRP
scFv fragment
产品细节
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抗原
See all IgG products
IgG
抗体类型
Recombinant Antibody
片段
scFv fragment
适用
All reactivities for IgG Secondaries
人
宿主
All hosts for IgG Secondaries
小鼠
克隆类型
All clonalities for IgG Secondaries
单克隆
标记
All conjugates for IgG Secondaries
HRP
应用范围
All applications for IgG Secondaries
ELISA, Western Blotting (WB), Dot Blot (DB)
原理
Anti-Human IgG scFv-HRP Antibody
特异性
Mouse variable regions (produced in Nicotiana benthamiana plants)
产品特性
This recombinantly produced secondary anti-human IgG scFv fused to HRP is optimised for western blot and ELISA use. The reddish/brown colour is common with HRP conjugated antibodies.
纯化方法
This product was purified using affinity chromatography (Ni-IMAC)
纯度
90-95 %
免疫原
Human IgG, whole molecule
Alternatives
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使用细节
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应用备注
Western blot: Suggested working dilution 1/1000-1/5000 to detect 1 μg antigen in 5 % milk. Suggested incubation conditions:1 hr at 37C/ 4C overnight.
限制
仅限研究用
贮存及处理
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状态
Liquid
浓度
1 mg/mL
缓冲液
Phosphate Buffered saline (0.1M) pH 7.4
储存条件
4 °C,-20 °C
储存方法
Short term: 2-4°C. Long term: -20°C
目标详细情况
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抗原
IgG
Abstract
IgG 产品
物质类
Antibody
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IgG
Recombinant 小鼠 anti-人 IgG Antibody (HRP) (ABIN7233210)