山羊 anti-大鼠 IgG Antibody (DyLight 549)
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- 抗原 See all IgG products
- IgG
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- DyLight 549
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 原理
- Dylight 549, Goat Anti-Rat IgG
- 特异性
- The antibody reacts with whole molecule rat IgG. It also reacts with heavy chains of rat IgG, and light chains of all other rat immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.
- 纯化方法
- Affinity purified using solid phase Rat IgG (H&L) with finally > 95% purity based on SDS-PAGE
- 免疫原
- Rat IgG whole molecule
- 亚型
- IgG
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Goat anti-Rat IgG (Heavy & Light Chain) antibody (FITC) - Preadsorbed
FACS, FM Polyclonal IgG FITC
Goat anti-Rat IgG (Fc Region) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Chicken anti-Rat IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Rat IgG (Heavy & Light Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Goat anti-Rat IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Rat IgG antibody (Cy3) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Cy3
Goat anti-Rat IgG (Heavy & Light Chain) antibody (beta-Gal) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG beta-Gal
Goat anti-Rat IgG (Heavy Chain) antibody (TRITC)ELISA, IHC, FACS Polyclonal IgG TRITC
Goat anti-Rat IgG (Heavy Chain) antibodyELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (PE)FACS Polyclonal IgG PE F(ab')2 fragment
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- 应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.
- 说明
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DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability. They are better than or comparable to the best fluorescent dyes from other companies. The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye, antibody activity, specificity, and cross-reactivity, and the optimal moles of dye per antibody. A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background. DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Liquid in PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative, 1 % BSA as stablizer and 50 % Glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Dylight 549 conjugated Goat Anti-Rat IgG (H+L) secondary antibody,Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.,Goat Anti-Rat IgG
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