山羊 anti-大鼠 IgG Antibody (Cy5) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Cy5
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应用范围
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)
- Supplier Product No.
- 612-110-120
- Supplier
- Rockland
- 原理
- Rat IgG (H&L) Antibody CY5 Conjugated Pre-Adsorbed
- 交叉反应 (详细)
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins
- 产品特性
- Goat Anti Rat IgG CY5 Conjugated Antibody, Goat Anti-Rat IgG Antibody CY5 Conjugation,Anti-Rat IgG (H&L) CY5 Antibody generated in goat detects reactivity to Rat IgG.
- 纯化方法
- Preadsorption: Pre-Adsorbed
- 免疫原
- Rat IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 7.4
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- 应用备注
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FLISA_Dilution: 1:10,000 - 1:50,000
Flow_Cytometry_Dilution: 1:500 - 1:2,500
IF_Microscopy_Dilution: 1:1,000 - 1:5,000
Western_Blot_Dilution: User Optimized
- 说明
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Anti-Rat IgG (H&L) CY5 Antibody has been tested by ELISA, dot blot, and western blot. This antibody is designed for flow cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG is conjugated to CY5.
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