山羊 anti-兔 IgG (Fc Region) Antibody (DyLight 680) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Fc Region
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- DyLight 680
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应用范围
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- 产品特性
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Synonyms: Goat Anti Rabbit IgG F(c) DyLight 680™ Conjugated Antibody, Goat Anti-Rabbit IgG Fc Fragment Antibody DyLight 680™ conjugation, Goat Anti Rabbit IgG Fc Antibody DyLight 680™ conjugated
Background: Anti-Rabbit IgG F(c) DyLight generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Rabbit IgG F(c) fragment
- 亚型
- IgG
- Labeling Ratio
- 2.8
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- 应用备注
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Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." in: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).
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Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." in: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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