山羊 anti-兔 IgG Antibody (DyLight 488)
(10 references)
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- 抗原 See all IgG products
- IgG
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- DyLight 488
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB), Multiplex Assay (MA)
- Supplier Product No.
- 611-141-002
- Supplier
- Rockland
- 原理
- Rabbit IgG (H&L) Secondary Antibody DyLight™ 488 Conjugated
- 产品特性
- Goat anti-Rabbit IgG Antibody DyLight™488 Conjugation, Goat anti-Rabbit IgG DyLight™ 488 Conjugated Antibody,Anti-Rabbit IgG (H&L) DyLight 488 Antibody generated in goat detects reactivity to Rabbit IgG.
- 纯化方法
- Conjugated Anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.
- 免疫原
- Rabbit IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 3.6
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Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&RUN, CUT&Tag Polyclonal IgG unconjugated
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC)WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)High-quality product from Rockland ELISA, IHC, WB, DB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 800)ELISA, WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Rabbit IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG Atto 488
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- 应用备注
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
- 说明
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Anti-Rabbit IgG (H&L) DyLight 488 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: FC, IHC, WB - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells." in: Scientific reports, Vol. 11, Issue 1, pp. 644, (2021) (PubMed).
: "Generation of Self-assembled Vascularized Human Skin Equivalents." in: Journal of visualized experiments : JoVE, Issue 168, (2021) (PubMed).
: "A platform for post-translational spatiotemporal control of cellular proteins." in: Synthetic biology (Oxford, England), Vol. 6, Issue 1, pp. ysab002, (2021) (PubMed).
: "Effects of TAZ on human dental pulp stem cell proliferation and migration." in: Molecular medicine reports, Vol. 15, Issue 6, pp. 4326-4332, (2018) (PubMed).
: "Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005041, (2017) (PubMed).
"Correction: Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." in: PLoS computational biology, Vol. 12, Issue 9, pp. e1005149, (2016) (PubMed).
: "Focused ultrasound and interleukin-4 receptor-targeted liposomal doxorubicin for enhanced targeted drug delivery and antitumor effect in glioblastoma multiforme." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 160, Issue 3, pp. 652-8, (2012) (PubMed).
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: "CD8+ T cell infiltration and proliferation in the brainstem during experimental cerebral malaria." in: CNS neuroscience & therapeutics, Vol. 30, Issue 3, pp. e14431, (2024) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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