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山羊 anti-兔 IgG Antibody (DyLight 800)

Name: 山羊 anti-兔 IgG Antibody (DyLight 800)
SKU: ABIN6699118
Availability: OutOfStock

Rockland 611-145-002 ELISA, WB, FLISA, FM, DB Polyclonal IgG DyLight 800

DyLight™ dyes can be used for two-color western blot detection with low background and high signal. (山羊 anti-兔 IgG Antibody (DyLight 800))

1 image

(67 references)

产品编号 ABIN6699118

Supplier Product No.: 611-145-002

发货至: 中国

Datasheet as PDF

抗原 See all IgG products

IgG
适用
720

692

593

465

393

240

208

176

145

137

112

106

105

101

82

58

47

28

27

16

15

10

8

5

5

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1
兔
宿主
1760

1549

569

238

112

111

32

31

21

18

15

11

11
山羊
克隆类型
3389

175

1
多克隆
标记
583

418

414

378

274

262

235

119

106

72

67

64

61

58

58

50

44

43

40

39

39

37

35

32

32

31

30

29

29

29

29

29

28

28

28

28

28

28

28

28

27

27

26

26

25

22

21

19

17

17

16
DyLight 800
应用范围
2032

1982

1835

1784

983

919

895

486

419

234

234

151

99

84

65

52

51

30

28

25

20

15

11

9

8

7

5

5

4

4

4

3

2

2

1

1

1

1

1

1

1

1
ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.

611-145-002

Supplier

Rockland

原理

Rabbit IgG (H&L) Antibody DyLight™ 800 Conjugated

产品特性

Goat anti-Rabbit IgG Antibody DyLight™800 Conjugation, Goat anti-Rabbit IgG DyLight™ 800 Conjugated Antibody,Anti-Rabbit IgG Antibody DyLight™800 generated in goat detects rabbit IgG.

纯化方法

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.

免疫原

Rabbit IgG whole molecule

亚型

IgG

Labeling Ratio

1.7
Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&RUN, CUT&Tag Polyclonal IgG unconjugated

Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC)
WB, FACS, FLISA, FM, DB Polyclonal IgG FITC

Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)
High-quality product from Rockland ELISA, IHC, WB, DB Polyclonal IgG HRP

Goat anti-Rabbit IgG (Whole Molecule) antibody (HRP)
ELISA, WB, DB Polyclonal IgG HRP

Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) - Preadsorbed
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Rabbit IgG antibody (DyLight 488)
WB, IF, FLISA, FM, DB, MA Polyclonal IgG DyLight 488

Goat anti-Rabbit IgG antibody (DyLight 800) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG DyLight 800

Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 647N

Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 550) - Preadsorbed
WB, FLISA, FM, DB Polyclonal IgG Atto 550

应用备注

FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized

说明

Anti-Rabbit IgG Antibody DyLight™800 has been tested by ELISA, dot blot, and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: EMSA, IHC, IP

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
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Yang, Zhou, Zhang, Wang, Wang, Cheng, Li, Jiang, Yang, Zhang: "The transcription factor HBP1 promotes ferroptosis in tumor cells by regulating the UHRF1-CDO1 axis." in: PLoS biology, Vol. 21, Issue 7, pp. e3001862, (2023) (PubMed).

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Cheng, Yang, Zhou, Wang, Zhang, Wang, Li, Jiang, Zhang: "HBP1 inhibits the development of type 2 diabetes mellitus through transcriptional activation of the IGFBP1 gene." in: Aging, Vol. 14, Issue 21, pp. 8763-8782, (2022) (PubMed).

Kitaoka, Smith, Straight, Heald: "Molecular conflicts disrupting centromere maintenance contribute to Xenopus hybrid inviability." in: Current biology : CB, Vol. 32, Issue 18, pp. 3939-3951.e6, (2022) (PubMed).

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抗原

IgG

Abstract

IgG 产品

物质类

Antibody

背景

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™800.