驴 anti-兔 IgG Antibody (DyLight 549) - Preadsorbed
(2 references)-
- 抗原 See all IgG products
- IgG
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适用
- 兔
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宿主
- 驴
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克隆类型
- 多克隆
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标记
- DyLight 549
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应用范围
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- 产品特性
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Synonyms: Donkey Anti-Rabbit IgG Antibody DyLight 549™ Conjugated, Donkey Anti Rabbit IgG DyLight 549™ Conjugated Antibody
Background: Anti-Rabbit IgG (H&L) DyLight 549 Antibody generated in donkey detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Rabbit IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 3.3
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Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&RUN, CUT&Tag Polyclonal IgG unconjugated
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Rabbit IgG antibody (HRP)ELISA, WB IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 425
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 488) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 488
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 647N
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG TRITC
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- 应用备注
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Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Anti-Rabbit IgG (H&L) DyLight 549 Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Protective Effect of ALA in Crushed Optic Nerve Cat Retinal Ganglion Cells Using a New Marker RBPMS." in: PLoS ONE, Vol. 11, Issue 8, pp. e0160309, (2017) (PubMed).
: "Ischemia induces different levels of hypoxia inducible factor-1α protein expression in interneurons and pyramidal neurons." in: Acta neuropathologica communications, Vol. 2, pp. 51, (2014) (PubMed).
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: "Protective Effect of ALA in Crushed Optic Nerve Cat Retinal Ganglion Cells Using a New Marker RBPMS." in: PLoS ONE, Vol. 11, Issue 8, pp. e0160309, (2017) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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