山羊 anti-兔 IgG Antibody (Cy3) - Preadsorbed
(2 references)-
- 抗原 See all IgG products
- IgG
-
适用
- 兔
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- Cy3
-
应用范围
- Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 产品特性
-
Synonyms: goat anti-Rabbit IgG CY3 Conjugated antibody, goat anti-Rabbit IgG antibody CY3 conjugation
Background: Anti-Rabbit IgG CY3 Conjugated Antibody generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to Cy3.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
-
Immunogen: Rabbit IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 10.1
-
Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&Tag, CUT&RUN Polyclonal IgG unconjugated
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC) - PreadsorbedFACS, FLISA, FM Polyclonal IgG FITC
Goat anti-Rabbit IgG antibody (HRP)ELISA, WB IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 425) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 425
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 488) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 488
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 647N
-
- 应用备注
-
Application Note: Anti-Rabbit IgG (H&L) Antibody CY3 Conjugated Pre-adsorbed antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
Western Blot Dilution: User Optimized
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
-
: "Antifibrotic, nephroprotective effects of paricalcitol versus calcitriol on top of ACE-inhibitor therapy in the COL4A3 knockout mouse model for progressive renal fibrosis." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 29, Issue 5, pp. 1012-9, (2014) (PubMed).
: "Nuclear receptor DHR4 controls the timing of steroid hormone pulses during Drosophila development." in: PLoS biology, Vol. 9, Issue 9, pp. e1001160, (2012) (PubMed).
-
: "Antifibrotic, nephroprotective effects of paricalcitol versus calcitriol on top of ACE-inhibitor therapy in the COL4A3 knockout mouse model for progressive renal fibrosis." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 29, Issue 5, pp. 1012-9, (2014) (PubMed).
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
-
