山羊 anti-小鼠 IgM Antibody (DyLight 680)
(1 reference)
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- 抗原 See all IgM products
- IgM
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- DyLight 680
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应用范围
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 610-144-007
- Supplier
- Rockland
- 原理
- Mouse IgM (mu chain) Antibody DyLight™ 680 Conjugated
- 产品特性
- Goat Anti-Mouse IgM (mu chain) Antibody DyLight 680™ Conjugated, Goat Anti Mouse IgM mu chain DyLight 680™ Conjugated Antibody,Anti-Mouse IgM DyLight antibody specifically detects mouse IgM.
- 纯化方法
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against other mouse heavy or light chain proteins.
- 免疫原
- Mouse IgM whole molecule
- 亚型
- IgG
- Labeling Ratio
- 2.1
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Goat anti-Mouse IgM (Heavy Chain) antibody (Biotin)
ELISA, FACS, IHC, WB Polyclonal IgG Biotin
Goat anti-Mouse IgM (Heavy Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Mouse IgM (Heavy Chain) antibodyELISA, FACS, IHC, WB Polyclonal IgG unconjugated
Goat anti-Mouse IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
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- 应用备注
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
- 说明
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The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: WB - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Interleukin 17A deficiency alleviates neuroinflammation and cognitive impairment in an experimental model of diabetic encephalopathy." in: Neural regeneration research, Vol. 17, Issue 12, pp. 2771-2777, (2022) (PubMed).
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: "Interleukin 17A deficiency alleviates neuroinflammation and cognitive impairment in an experimental model of diabetic encephalopathy." in: Neural regeneration research, Vol. 17, Issue 12, pp. 2771-2777, (2022) (PubMed).
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Mouse IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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