兔 anti-小鼠 IgG3 (Heavy Chain) Antibody (DyLight 549)
(1 reference)
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- 抗原 See all IgG3 products
- IgG3
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抗原表位
- Heavy Chain
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- DyLight 549
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应用范围
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 610-442-043
- Supplier
- Rockland
- 原理
- Mouse IgG3 (Gamma 3 chain) Antibody DyLight™ 549 Conjugated
- 产品特性
- Rabbit Anti-Mouse IgG3 (Gamma 3 chain) DyLight 549™ Conjugated Antibody, Rabbit Anti Mouse IgG3 Antibody DyLight 549™ Conjugation,Anti-Mouse IgG3 DyLight 549 Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain).
- 免疫原
- Mouse IgG3 heavy chain
- 亚型
- IgG
- Labeling Ratio
- 3.1
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Goat anti-Mouse IgG3 antibody (HRP)
ELISA, FLISA Polyclonal IgG HRP
Goat anti-Mouse IgG3 (Heavy Chain) antibody (HRP)ELISA, WB, IHC Polyclonal IgG HRP
Goat anti-Mouse IgG3 antibodyELISA, FLISA Polyclonal IgG unconjugated
Goat anti-Mouse IgG3 (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Mouse IgG3 antibody (FITC)ELISA, FLISA Polyclonal IgG FITC
Rat anti-Mouse IgG3 antibodyELISA, FACS, FLISA Monoclonal SB76b IgG2a unconjugated
Goat anti-Mouse IgG3 (Heavy Chain) antibodyELISA, WB, IHC, FACS Polyclonal IgG unconjugated
Rat anti-Mouse IgG3 antibody (Biotin)ELISA, FACS, FLISA Monoclonal SB76b IgG2a Biotin
Rat anti-Mouse IgG3 antibody (HRP)ELISA, FACS, FLISA Monoclonal SB76b IgG2a HRP
Rat anti-Mouse IgG3 antibody (Alkaline Phosphatase (AP))ELISA, FACS, FLISA Monoclonal SB76b IgG2a Alkaline Phosphatase (AP)
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- 应用备注
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
- 说明
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This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, Multiplex - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate." in: Nature communications, Vol. 6, pp. 7673, (2016) (PubMed).
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: "9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate." in: Nature communications, Vol. 6, pp. 7673, (2016) (PubMed).
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- 抗原
- IgG3
- Abstract
- IgG3 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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