山羊 anti-小鼠 IgG Antibody (DyLight 680) - Preadsorbed
(7 references)
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- 抗原 See all IgG products
- IgG
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- DyLight 680
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应用范围
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 610-144-121
- Supplier
- Rockland
- 原理
- Mouse IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed
- 交叉反应 （详细）
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins
- 产品特性
- Goat Anti-Mouse IgG Secondary Antibody DyLight™680 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™680 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™680 conjugated secondary antibody,Anti-Mouse IgG DyLight 680 Antibody generated in goat detects reactivity to Mouse IgG.
- 纯化方法
- Preadsorption: Pre-Adsorbed
- 免疫原
- Mouse IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 2.1
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM, DB Polyclonal IgG TRITC
Goat anti-Mouse IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
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- 应用备注
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
- 说明
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This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, IHC, Microarray, Multiplex, WB - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "FUS ALS neurons activate major stress pathways and reduce translation as an early protective mechanism against neurodegeneration." in: Cell reports, Vol. 42, Issue 2, pp. 112025, (2023) (PubMed).
: "TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion." in: Molecular oncology, Vol. 14, Issue 10, pp. 2609-2628, (2021) (PubMed).
: "Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity." in: eLife, Vol. 9, (2021) (PubMed).
: "Caffeine improves bladder function in diabetic rats via a neuroprotective effect." in: Experimental and therapeutic medicine, Vol. 21, Issue 5, pp. 501, (2021) (PubMed).
: "Local Fatty Acid Channeling into Phospholipid Synthesis Drives Phagophore Expansion during Autophagy." in: Cell, Vol. 180, Issue 1, pp. 135-149.e14, (2020) (PubMed).
: "Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin." in: Nature communications, Vol. 11, Issue 1, pp. 501, (2020) (PubMed).
: "Hallmarks of Alzheimer's Disease in Stem-Cell-Derived Human Neurons Transplanted into Mouse Brain." in: Neuron, Vol. 93, Issue 5, pp. 1066-1081.e8, (2017) (PubMed).
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: "FUS ALS neurons activate major stress pathways and reduce translation as an early protective mechanism against neurodegeneration." in: Cell reports, Vol. 42, Issue 2, pp. 112025, (2023) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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