山羊 anti-小鼠 IgG Antibody (DyLight 680)
(31 references)
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- 抗原 See all IgG products
- IgG
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- DyLight 680
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应用范围
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)
- Supplier Product No.
- 610-144-002
- Supplier
- Rockland
- 原理
- Mouse IgG (H&L) Antibody Dylight™ 680 Conjugated
- 产品特性
- Goat Anti-Mouse IgG Secondary Antibody DyLight™680 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™680 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™680 conjugated secondary antibody,Anti-Mouse IgG DyLight 680 Antibody generated in goat detects reactivity to Mouse IgG.
- 纯化方法
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This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.
- 免疫原
- Mouse IgG, whole molecule
- 亚型
- IgG
- Labeling Ratio
- 2.6
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Goat anti-Mouse IgG antibody (DyLight 800)
WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG antibody (DyLight 488) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 488
Goat anti-Mouse IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (TRITC) - PreadsorbedFACS, FLISA, FM, DB Polyclonal IgG TRITC
Rabbit anti-Mouse IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Rabbit anti-Mouse IgG antibody (HRP) - PreadsorbedELISA, WB, IHC (fro), IHC (p), IHC (rs) Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
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- 应用备注
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FLISA_Dilution: >1:20,000
IF_Microscopy_Dilution: >1:5,000
Western_Blot_Dilution: >1:10,000
Other: User Optimized
- 说明
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Anti-Mouse IgG DyLight 680 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, IHC, IP - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Gesicle-Mediated Delivery of CRISPR/Cas9 Ribonucleoprotein Complex for Inactivating the HIV Provirus." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 27, Issue 1, pp. 151-163, (2019) (PubMed).
: "Arkadia/RNF111 is a SUMO-targeted ubiquitin ligase with preference for substrates marked with SUMO1-capped SUMO2/3 chain." in: Nature communications, Vol. 10, Issue 1, pp. 3678, (2019) (PubMed).
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: "MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse model of heteroplasmic mtDNA mutation." in: Nature medicine, Vol. 24, Issue 11, pp. 1696-1700, (2019) (PubMed).
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: "Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons." in: Glia, Vol. 72, Issue 8, pp. 1374-1391, (2024) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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