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驴 anti-山羊 IgG Antibody (DyLight 549) - Preadsorbed

Rockland 605-742-125 WB, FLISA, FM, DB Polyclonal IgG DyLight 549
Rockland
产品编号 ABIN6698864
Supplier Product No.: 605-742-125
发货至: 中国
  • 抗原 See all IgG products
    IgG
    适用
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    山羊
    宿主
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    • 11
    克隆类型
    • 3386
    • 184
    • 1
    多克隆
    标记
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    DyLight 549
    应用范围
    • 1961
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    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)
    Supplier Product No.
    605-742-125
    Supplier
    Rockland
    原理
    Goat IgG (H&L) Antibody DyLight™ 549 Conjugated Pre-Adsorbed
    交叉反应 (详细)
    Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins
    产品特性
    Donkey anti Goat IgG Antibody DyLight™ 549 Conjugated Pre-Adsorbed,Anti-Goat IgG DyLight Antibody generated in donkey detects goat IgG.
    纯化方法
    Preadsorption: Pre-Adsorbed
    免疫原
    Goat IgG whole molecule
    亚型
    IgG
    Labeling Ratio
    5.2
  • 应用备注

    FLISA_Dilution: >1:20,000

    IF_Microscopy_Dilution: >1:5,000

    Western_Blot_Dilution: >1:10,000

    Other: User Optimized

    说明

    Anti-Goat IgG DyLight 549 Antibody has been tested by dot blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
    Suggested Applications: IF, Multiplex

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Scoles, Pflieger, Thai, Hansen, Dansithong, Pulst: "ETS1 regulates the expression of ATXN2." in: Human molecular genetics, Vol. 21, Issue 23, pp. 5048-65, (2013) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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