山羊 anti-小鼠 IgG Antibody (Cy2) - Preadsorbed
-
- 抗原 See all IgG products
- IgG
- 片段
- F(ab')2 fragment
-
适用
- 小鼠
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- Cy2
-
应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 产品特性
-
Synonyms: Goat F(ab')2 Anti-Mouse IgG Antibody CY2 Conjugation, Goat Fab2 Anti-Mouse IgG CY2 Conjugated Antibody
Background: F(ab')2 Anti-Mouse IgG (H&L) Cy2 Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
-
Immunogen: Mouse IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 1.9
-
-
- 应用备注
-
Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
-