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小鼠 anti-人 IgM Antibody (CF®594)

FACS, IF, IHC (f) Monoclonal DA4-4 IgG1 kappa CF®594
产品编号 ABIN6154959
发货至: 中国
  • 抗原 See all IgM products
    IgM
    适用
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    小鼠
    克隆类型
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    单克隆
    标记
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    CF®594
    应用范围
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    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    原理
    Mouse Monoclonal anti-Human IgM Immunoglobulin (DA4-4), CF594 Conjugate
    产品特性
    This antibody recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    Heavy chain of human IgM
    克隆位点
    DA4-4
    亚型
    IgG1 kappa
  • 应用备注
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    说明

    293T, Raji or hPBL cells. Tonsil or Spleen

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect from light
  • 抗原
    IgM
    别名
    IgM Immunoglobulin (IgM 产品)
    物质类
    Antibody
    背景
    AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region
    分子量
    50-75 kDa
    基因ID
    3507, 510635
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