(1 reference)-
- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 人
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宿主
- 兔
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标记
- DyLight 594
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
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Rabbit anti-human IgG (H&L) - Affinity Pure, DyLight 594 Conjugate.
Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm).
Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody. - 纯化方法
- Affinity purified using solid phase mouse IgM (H&L)
- 纯度
- > 95 % based on SDS-PAGE
- 免疫原
- Purified human IgG, whole molecule
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Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Mouse anti-Human IgG (Fc Region) antibody (PE)ELISA, FACS Monoclonal JDC-10 IgG1 PE
Mouse anti-Human IgG (Fab Region) antibody (PE)FACS Monoclonal 4A11 IgG2b PE
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Goat anti-Human IgG (Fc Region) antibody (PE) - PreadsorbedFACS, FM Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgG antibody (HRP) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Human IgG (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
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- 应用备注
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This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications - 说明
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Country of Origin: Rabbit serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- 1.0 mg/mL
- 缓冲液
- 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
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Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. - 储存条件
- 4 °C
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: "The cytoskeletal/non-muscle isoform of alpha-actinin is phosphorylated on its actin-binding domain by the focal adhesion kinase." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 28676-85, (2001) (PubMed).
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: "The cytoskeletal/non-muscle isoform of alpha-actinin is phosphorylated on its actin-binding domain by the focal adhesion kinase." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 28676-85, (2001) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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