(3 references)-
- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 人
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宿主
- 山羊
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标记
- DyLight 650
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
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Goat anti-human IgG (H&L) - Affinity Pure, DyLight 650 Conjugate.
Fluorphore: DyLight 650 (Ex = 652 nm, Em = 672 nm).
Fluor Protein Ratio: Moles DyLight 650 per Mole Antibody. - 纯化方法
- Affinity purified using solid phase human IgE (H&L)
- 纯度
- > 95 % based on SDS-PAGE
- 免疫原
- Purified human IgG, whole molecule
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Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Mouse anti-Human IgG (Fc Region) antibody (PE)ELISA, FACS Monoclonal JDC-10 IgG1 PE
Mouse anti-Human IgG (Fab Region) antibody (PE)FACS Monoclonal 4A11 IgG2b PE
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Goat anti-Human IgG (Fc Region) antibody (PE) - PreadsorbedFACS, FM Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgG antibody (HRP) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Human IgG (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
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- 应用备注
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This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications - 说明
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Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
- 浓度
- 1.0 mg/mL
- 缓冲液
- 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 储存条件
- 4 °C
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: "Signal-binding specificity of the mu4 subunit of the adaptor protein complex AP-4." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 13145-52, (2001) (PubMed).
: "AP-4, a novel protein complex related to clathrin adaptors." in: The Journal of biological chemistry, Vol. 274, Issue 11, pp. 7278-85, (1999) (PubMed).
: "Characterization of a fourth adaptor-related protein complex." in: Molecular biology of the cell, Vol. 10, Issue 8, pp. 2787-802, (1999) (PubMed).
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: "Signal-binding specificity of the mu4 subunit of the adaptor protein complex AP-4." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 13145-52, (2001) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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