兔 anti-猴 IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)
- 产品特性
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Synonyms: rabbit Anti-Monkey IgG peroxidase Conjugated Antibody, rabbit Anti-Monkey IgG Antibody peroxidase Conjugation, rabbit Anti-Monkey IgG HRP Conjugated Antibody
Background: Anti-Monkey IgG (H&L) HRP antibody generated in rabbit detects specifically monkey IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This peroxidase conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform western-blot, immunoprecipitation and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
- 纯化方法
- Preadsorption: immunoaffinity chromatography using Monkey IgG coupled to agarose beads
- 免疫原
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Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Monkey IgG (Chain gamma) antibody (HRP)
ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Goat anti-Monkey IgG (Heavy Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Monkey IgG (H&L) HRP conjugated Antibody is suitable for use in Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods. Specific conditions for reactivity and signal detection should be optimized by the end user.
Western Blot Dilution: 1:10,000 - 1:60,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:50,000 - 1:100,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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