兔 anti-猴 IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))
Rockland 617-405-002
ELISA, IHC, WB
Polyclonal
IgG
Alkaline Phosphatase (AP)
1 image
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- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 猴
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Alkaline Phosphatase (AP)
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 617-405-002
- Supplier
- Rockland
- 原理
- Monkey IgG (H&L) Antibody Alkaline Phosphatase
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-alkaline phosphatase, Monkey IgG and Monkey Serum.
- 产品特性
- Anti-Monkey IgG (H&L) HRP antibody generated in rabbit detects specifically monkey IgG heavy and light chains.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.
Immunogen Type: Native Protein
- 亚型
- IgG
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Goat anti-Monkey IgG antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP
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- 应用备注
- Application Note: Anti-Monkey IgG (H&L) ALP conjugated Antibody has been tested by ELISA and is suitable for use in Western Blotting, Immunohistochemistry, ELISA as well as other antibody detection methods. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store secondary antibody at 4° C before opening. DO NOT FREEZE. Alkaline Phosphatase conjugated antibodies are stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
- Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This alkaline phosphatase conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform western blots, ELISAs, and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
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