山羊 anti-大鼠 IgG2ab, Subclass Specific Antibody (HRP)
IP, IF, DB, IHC (fro), ID, EIA, WB
Polyclonal
IgG
HRP
-
- 抗原 See all IgG2ab products
- IgG2ab (IgG2ab, Subclass Specific)
- 适用
- 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
-
标记
- HRP
-
应用范围
- Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Western Blotting (WB)
- 产品特性
- Molar Ratio: Peroxidase/IgG aprox. 1.0
- 纯化方法
- Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.
- 免疫原
- Pools of purified homogenous IgG2a and IgG2b isolated from rat sera. Freund's complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
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- 应用备注
- Optimal working dilutions should be determined experimentally by the investigator.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 溶解方式
- Restore with 1,0 mL distilled water. Working dilutions should be prepared by adding sterile PBS, pH 7.2
- 浓度
- 10.0 mg/mL
- 缓冲液
- PBS, pH 7.2 without preservatives and foreign proteins
- 储存液
- Without preservative
- 注意事项
-
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -28 °C for longer.
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- 抗原
- IgG2ab (IgG2ab, Subclass Specific)
- 别名
- IgG2ab (IgG2ab 产品)
- 背景
- Synonyms: Rat Immunoglobulin G2ab
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