兔 anti-小鼠 lambda (Light Chain) Antibody (Biotin) - Preadsorbed
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- 抗原
- lambda
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抗原表位
- Light Chain
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Mouse lambda light chain
- 亚型
- IgG
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Rat anti-Mouse lambda antibody (PE)
FACS, ELISA Monoclonal JC5-1 IgG2b PE
Rat anti-Mouse lambda antibodyFACS, ELISA Monoclonal JC5-1 IgG2b unconjugated
Rat anti-Mouse lambda antibody (Biotin)FACS, ELISA Monoclonal JC5-1 IgG2b Biotin
Rat anti-Mouse lambda antibody (FITC)FACS, ELISA Monoclonal JC5-1 IgG2b FITC
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- lambda
- 别名
- lambda )
- 背景
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Synonyms: Rabbit Anti-Mouse λ (lambda chain) Biotin Conjugated Antibody, Rabbit Anti Mouse lambda Antibody Biotin conjugation
Background: Anti-Mouse λ (lambda chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse lambda light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG lambda is conjugated to biotin.
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