驴 anti-人 IgM (Fc5mu Region) Antibody (PE) - Preadsorbed
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- 抗原 See all IgM products
- IgM
- 片段
- F(ab')2 fragment
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抗原表位
- Fc5mu Region
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适用
- 人
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宿主
- 驴
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克隆类型
- 多克隆
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标记
- PE
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Human IgM Fc5mu fragment
- 亚型
- IgG
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Mouse anti-Human IgM antibody (FITC)
FACS Monoclonal CH2 IgG1 FITC
Mouse anti-Human IgM antibody (APC)FACS Monoclonal CH2 IgG1 APC
Goat anti-Human IgM antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Mouse anti-Human IgM antibody (PE)FACS Monoclonal CH2 IgG1 PE
Mouse anti-Human IgM antibody (iFluor™647)GMP Grade FACS Monoclonal M-2E6 IgG1 iFluor™647
Mouse anti-Human IgM antibody (mFluor™450)GMP Grade FACS Monoclonal M-2E6 IgG1 mFluor™450
Mouse anti-Human IgM antibody (PE-Cy7)GMP Grade FACS Monoclonal M-2E6 IgG1 PE-Cy7
Goat anti-Human IgM antibody (FITC)ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human IgM (Heavy Chain) antibody (FITC)ELISA, FACS, IHC Polyclonal IgG FITC
Goat anti-Human IgM (Heavy Chain) antibody (TRITC)ELISA, FACS, IHC Polyclonal IgG TRITC
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- 应用备注
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Application Note: F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.
Flow Cytometry Dilution: 1:100 - 1:250
IF Microscopy Dilution: 1:100 - 1:250
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 0.5 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C
- 储存方法
- Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
- 有效期
- 6 months
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
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Synonyms: Donkey F(ab')2 anti-Human IgM Fc5u Antibody Phycoerythrin Conjugation, Donkey F(ab')2 anti-Human IgM Fc5u PE Conjugated Antibody
Background: F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody generated in donkey detects specifically the Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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