山羊 anti-人 lambda (Light Chain) Antibody (HRP) - Preadsorbed
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- 抗原
- lambda
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抗原表位
- Light Chain
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 交叉反应
- 人
- 产品特性
- Anti-Human lambda chain peroxidase conjugated antibody generated in goat detects specifically human lambda chain. This secondary peroxidase conjugated antibody anti-Human lambda chain is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Anti-Human lambda chain was produced by repeated immunization with human lambda light chain in goat.
Immunogen Type: Native Protein
- 亚型
- IgG
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Mouse anti-Human lambda antibody (PE)
FACS, ELISA Monoclonal JDC-12 IgG1 PE
Goat anti-Human lambda (Chain lambda) antibodyFACS, ELISA Polyclonal IgG unconjugated F(ab')2 fragment
Mouse anti-Human lambda (Chain lambda) antibodyFACS, ELISA Monoclonal JDC-12 IgG1 unconjugated
Goat anti-Human lambda (Chain lambda) antibody (Biotin)FACS, ELISA Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Human lambda (Chain lambda) antibody (FITC)FACS, ELISA Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human lambda (Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Mouse anti-Human lambda (Chain lambda) antibody (Biotin)FACS, ELISA Monoclonal JDC-12 IgG1 Biotin
Goat anti-Human lambda (Chain lambda) antibody - PreadsorbedFACS, ELISA Polyclonal IgG unconjugated F(ab')2 fragment
Mouse anti-Human lambda antibody (FITC)FACS, ELISA Monoclonal JDC-12 IgG1 FITC
Goat anti-Human lambda antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP Fab fragment
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- 应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: This secondary peroxidase conjugated antibody anti-Human lambda chain is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Human lambda chain Antibody peroxidase conjugated has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:16,000 to 1:64,000 is suggested for this product.
ELISA Dilution: 1:10,000 - 1:50,000
Western Blot Dilution: 1:1,000 - 1:10,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- lambda
- 别名
- lambda )
- 背景
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Synonyms: goat anti-Human λ (lambda chain) Antibody peroxidase Conjugated, goat anti-Human lambda chain HRP Conjugated Antibody
Background: The anti-Human λ (lambda chain) Antibody detects the lambda chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG lambda is conjugated to peroxidase.
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