山羊 anti-人 IgA Antibody (FITC)
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- 抗原 See all IgA products
- IgA
- 片段
- F(ab')2 fragment
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- Supplier Product No.
- 709-1206
- Supplier
- Rockland
- 原理
- F(ab')2 Human IgA (alpha chain) Antibody Fluorescein Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Human IgA and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other human heavy or light chain isotypes.
- 产品特性
- F(ab')2 Anti-Human IgG F(ab')2 Antibody generated in goat detects F(ab')2 from human. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
- 免疫原
- Optional[Immunogen]: Human IgA alpha heavy chain
- 亚型
- IgG
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Goat anti-Human IgA antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgA antibodyELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment
Goat anti-Human IgA (Heavy Chain) antibody (FITC)ELISA, FACS Polyclonal IgG FITC
Goat anti-Human IgA (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
Goat anti-Human IgA (Heavy Chain) antibody (Biotin)ELISA, WB, FACS Polyclonal IgG Biotin
Goat anti-Human IgA antibody (FITC)ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment
Goat anti-Human IgA antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Human IgA (Heavy Chain) antibody (Texas Red (TR))ELISA, FACS Polyclonal IgG Texas Red (TR)
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- 应用备注
- Application Note: F(ab')2 Anti-Human IgA Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500-1:2,500 IF Microscopy Dilution: 1:1,000-1:5,000 Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- 浓度
- 1.5 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.05 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgA
- Abstract
- IgA 产品
- 物质类
- Antibody
- 背景
- F(ab')2 Anti-Human IgA Fluorescein Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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